DNA Repair, Part B

Chapter 1 <br>Overproduction and purification of RFC-related clamp loaders and PCNA-related clamps from Saccharomyces cerevisiae <br>Chapter 2 <br>Functional Assays for Replication Protein A (RPA) <br>Chapter 3 <br>Human DNA Ligases I, III, and IV – purification and new specific assays for these enzymes. <br>Chapter 4 <br>Enzymatic Mechanism of the WRN Helicase/Nuclease <br>Chapter 5 <br>Analysis of the DNA Unwinding Activity of RECQ Family Helicases <br>Chapter 6 <br>Characterization of Checkpoints Responses to DNA damage in Saccharomyces cerevisiae: Basic Protocols <br>Chapter 7 <br>Recruitment of ATR-ATRIP, Rad17, and 9-1-1 Complexes to DNA Damage <br>Chapter 8 <br>Multiple approaches to study S. cerevisiae Rad9, a prototypical checkpoint protein <br>Chapter 9 <br>Methods for studying adaptation to the DNA damage checkpoint in yeast <br>Chapter 10 <br>DNA damage-induced phosphorylation of Rad55 protein as a sentinel for DNA damage checkpoint activation in S. cerevisiae <br>Chapter 11 <br>Methods for studying Mutagenesis and Checkpoints in Schizosaccharomyces pombe <br>Chapter 12 <br>Methods for Determining Spontaneous Mutation Rates <br>Chapter 13 <br>Genomic Approaches for Identifying DNA Damage Response Pathways in Saccharomyces cerevisiae <br>Chapter 14 <br>Techniques for y-H2AX detection <br>Chapter 15 <br>Methods for studying the cellular response to DNA damage: influence of the Mre11 complex on chromosome metabolism <br>Chapter 16 <br>Detecting repair intermediates in vivo: effects of DNA damage response genes on single stranded DNA accumulation at uncapped telomeres in budding yeast <br>Chapter 17 <br>Analysis of Non-B DNA Structure at Chromosomal Sites in the Mammalian Genome <br>Chapter 18 <br>Detection and Structural Analysis of R-Loops <br>Chapter 19 <br>The delitto perfetto approach to in vivo site-directed mutagenesis and chromosome rearrangements with synthetic oligonucleotides in yeast <br>Chapter 20 <br>Assays for transcriptional mutagenesis in active genes <br>Chapter 21 <br>Methods for studying chromatin assembly coupled to DNA repair <br>Chapter 22 <br>Structure-fucntion analysis of SWI2/SNF2 enzymes <br>Chapter 23 <br>Genomic Approach for the Understanding of Dynamic Aspect of Chromosome Behavior <br>Chapter 24 <br>Measurement of Chromosomal DNA Single-Strand Breaks and Replication Fork Progression Rates <br>Chapter 25 <br>Monitoring DNA replication following UV-induced damage in Escherichia coli <br>Chapter 26 <br>Methods to study replication fork collapse in budding yeast <br>Chapter 27 <br>Analysis of Gross-Chromosomal Rearrangements in Saccharomyces cerevisiae <br>Chapter 28 <br>Formation and processing of stalled replication forks – utility of two-dimensional agarose gels <br>Chapter 29 <br>Poly (ADP-ribose) polymerase-1 activation during DNA damage and repair