Charles Rupprecht,
Thirumeni Nagarajan
Elsevier Science
e druk, 2015
9780128019191
Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention, Volume 2
Specificaties
Gebonden, blz.
|
Engels
Elsevier Science |
2015
ISBN13: 9780128019191
Rubricering
Levertijd ongeveer 9 werkdagen
Gratis verzonden
Samenvatting
Laboratory Techniques in Rabies Diagnosis, Research and Prevention provides a basic understanding of the current trends in rabies. It establishes a new facility for rabies surveillance, vaccine and antibody manufacturing. It offers clarity about the choice of laboratory methods for diagnosis and virus typing, of systems for producing monoclonal and polyclonal antibodies and of methods for testing potency of vaccines and antibodies.The book covers advancements in the classical methods described as well as recent methods and approaches pertaining to rabies diagnosis and research.
Specificaties
Inhoudsopgave
<p>I. Foreword<br>II. Introduction<br>III. Rabies Diagnosis<br>a) Demonstration of virus<br>i) Electron microscopy<br>ii) Virus isolation in animals, the mouse inoculation test (MIT)<br>iii) Virus isolation in cell culture, the rapid tissue culture inoculation test (RTCIT)<br>iv) Rat basophilic leukemia cell sensor<br>b) Demonstration of viral subunits and antigens<br>i) Purification of rabies viral subunits<br>ii) Preparation of fluorescent antibody conjugate in rabbits<br>iii) Preparation of fluorescent antibody conjugate in goats<br>iv) DFA (postmortem)<br>v) Skin biopsy<br>vi) Immuno histochemistry<br>c) Demonstration of viral nucleic acids<br>i) Gel based PCR<br>ii) In situ PCR<br>d) Demonstration of viral antibodies<br>1. Binding Assays<br>i) ELISA - Traditional<br>ii) IFA<br>iii) Counter Immunoelectrophoresis<br>2. Functional Assays<br>i) Mouse neutralization test<br>ii) RFFIT<br>iii) FAVN<br>e) Typing/differentiation of lyssaviruses<br>i) Antigenic typing by MAbs</p> <p>IV. Rabies Biologics<br>i) Rabies vaccines for humans or other animals<br>1. Purified chick embryo cell vaccine<br>2. Purified Vero Cell Rabies Vaccine<br>3. In vivo NIH potency testing of rabies vaccines<br>9. In vitro Potency testing of rabies vaccines<br>ii) Rabies antibodies/fragments<br>1. Production of polyclonal rabies immune globulin in humans<br>2. Production of polyclonal antibodies in horses<br>3. Production of polyclonal antibodies in birds<br>4. Production of full length human monoclonal antibodies using transgenic mice</p> <p>V. Development of Anti-Viral Approaches</p> <p>VI. Appendix<br>i. List of biologics recommended by WHO for human rabies prophylaxis</p>