Isotope Labeling of Biomolecules – Applications

Nuclear Magnetic Resonance Spectroscopy<br>1. Application of Natural Isotopic Abundance 1H-13C and 1H-15N Correlated Two- Dimensional NMR for Evaluation of the Structure of Protein Therapeutics<br>Luke W. Arbogast, Robert G. Brinson and John P. Marino<br>2. A Semi-Automated Assignment Protocol for Methyl Group Side-Chains in Large Proteins<br>Jonggul Kim, Yingjie Wang, Geoffrey Li and Gianluigi Veglia<br>3. 19F-site Specific Labeled Nucleotides for Nucleic Acid Structural Analysis by NMR<br>Lincoln G. Scott and Mirko Hennig<br>4. Applying Thymine Isostere 2,4-Difluoro-5-methylbenzene as a NMR Assignment Tool and Probe of Homopyrimidine/Homopurine Tract Structural Dynamics<br>Robert G. Brinson, Jennifer T. Miller, Jason D. Kahn, Stuart F. J. Le Grice and John P. Marino<br>Scattering Techniques <br>5. Biomolecular Deuteration for Neutron Structural Biology and Dynamics<br>Michael Haertlein, Martine Moulin, Juliette M. Devos, Valerie Laux, Orla Dunne, V. Trevor Forsyth<br>6. Deuterium Labeling Together with Contrast Variation Small-angle Neutron Scattering Suggests How Skp Captures and Releases Unfolded Outer Membrane Proteins<br>Nathan R. Zaccai, Clifford W. Sandlin, James T. Hoopes, Joseph E. Curtis, Patrick J. Fleming, Karen G. Fleming and Susan Krueger<br>7. Deuteration in Biological Neutron Reflectometry<br>Frank Heinrich<br>8. Deuterium Labelling Strategies for Creating Contrast in Structure-Function Studies of Model Bacterial Outer Membranes Using Neutron Reflectometry <br>Anton P. Le Brun, Luke A. Clifton, Stephen A. Holt, Peter J. Holden, and Jeremy H. Lakey<br>9. Essential Strategies for Revealing Nanoscale Protein Dynamics By Neutron Spin Echo Spectroscopy<br>David J. E. Callaway and Zimei Bu<br>Mass Spectrometry<br>10. Method for the Determination of 15N Incorporation Percentage in Labeled Peptides and Proteins<br>Eric L. Kilpatrick<br>11. QconCAT: Internal Standard for Protein Quantification<br>Kerry Bauer Scott, Illarion V. Turko and Karen W. Phinney<br>12. Production, Purification and Characterization of 15N-Labeled DNA Repair Proteins as Internal Standards for Mass Spectrometric Measurements<br>Prasad T. Reddy, Pawel Jaruga, Bryant C. Nelson, Mark S. Lowenthal, Ann-Sofie Jemth, Olga Loseva, Erdem Coskun, Thomas Helleday and Miral Dizdaroglu<br>Hydrogen-Deuterium Exchange Mass Spectrometry<br>13. Hydrogen Exchange Mass Spectrometry<br>Leland Mayne<br>14. Mapping Protein-Ligand Interactions with Proteolytic Fragmentation, Hydrogen/Deuterium Exchange-Mass Spectrometry<br>Elyssia S. Gallagher and Jeffrey W. Hudgens<br>15. Isotope Labeling of Biomolecules “Structural analysis of viruses by HDX-MS"<br>Miklos Guttman and Kelly K. Lee