Molecular Characterization of Autophagic Responses Part A
Specificaties
Gebonden, blz.
|
Engels
Elsevier Science |
2017
ISBN13: 9780128096758
Rubricering
Onderdeel van serie
Methods in Enzymology
Levertijd ongeveer 9 werkdagen
Gratis verzonden
Samenvatting
Molecular Characterization of Autophagic Responses, Part A, presents a collection of methods for the qualitative and quantitative evaluation of virtually all the morphological, biochemical, and functional manifestations of autophagy, in vitro, ex vivo and in vivo, in organisms as distant as yeast and man.
Autophagy is an evolutionarily conserved mechanism for the lysosomal degradation of superfluous or dangerous cytoplasmic entities, and plays a critical role in the preservation of cellular and organismal homeostasis. Monitoring the biochemical processes that accompany autophagy is fundamental for understanding whether autophagic responses are efficient or dysfunctional.
Specificaties
ISBN13:9780128096758
Taal:Engels
Bindwijze:Gebonden
Uitgever:Elsevier Science
Serie:Methods in Enzymology
Inhoudsopgave
<p>1. Correlative Live Cell and Super Resolution Imaging of Autophagosome Formation<br>2. Quantifying Autophagic Structures in Mammalian Cells Using Confocal Microscopy<br>3. The Use of DQ-BSA to Monitor the Turnover of Autophagy-Associated Cargo<br>4. Turnover of Lipidated LC3 and Autophagic Cargoes in Mammalian Cells<br>5. High-Throughput Quantification of GFP-LC3<SUP>+</SUP> Dots by Automated Fluorescence Microscopy<br>6. Use of pHlurorin-mKate2-human LC3 to Monitor Autophagic Responses<br>7. Production of Human ATG Proteins for Lipidation Assays<br>8. Investigating Structure and Dynamics of Atg8 Family Proteins<br>9. Methods for Studying Interactions Between Atg8/LC3/GABARAP and LIR-Containing Proteins<br>10. Assessment of Posttranslational Modifications of ATG proteins<br>11. Tagged ATG8-Coding Constructs for the In Vitro and In Vivo Assessment of ATG4 Activity<br>12. Measurement of the Activity of the Atg4 Cysteine Proteases<br>13. Crystallographic Characterization of ATG Proteins and Their Interacting Partners<br>14. Dynamics of Atg5–Atg12–Atg16L1 Aggregation and Deaggregation<br>15. Fluorescent FYVE Chimeras to Quantify PtdIns3P Synthesis During Autophagy<br>16. Quantification of Phosphatidylinositol Phosphate Species in Purified Membranes<br>17. Mass Assays to Quantify Bioactive PtdIns3P and PtdIns5P During Autophagic Responses<br>18. Fluorescence-Based Assays to Analyse Phosphatidylinositol 5-Phosphate in Autophagy<br>19. Ultrastructural Characterization of Phagophores Using Electron Tomography on Cryoimmobilized and Freeze Substituted Samples<br>20. A Simple Cargo Sequestration Assay for Quantitative Measurement of Nonselective Autophagy in Cultured Cells<br>21. In Vitro Reconstitution of Autophagosome–Lysosome Fusion<br>22. In Vitro Reconstitution of Atg8 Conjugation and Deconjugation<br>23. Study of ULK1 Catalytic Activity and Its Regulation<br>24. Evaluating the mTOR Pathway in Physiological and Pharmacological Settings<br>25. Methods to Study the BECN1 Interactome in the Course of Autophagic Responses<br>26. In Vitro Characterization of VPS34 Lipid Kinase Inhibition by Small Molecules<br>27. Methods to Study Lysosomal AMPK Activation<br>28. Allosteric Modulation of AMPK Enzymatic Activity: In Vitro Characterization<br>29. Assessing the Catalytic Activity of Transglutaminases in the Context of Autophagic Responses</p>