Measurement and Analysis of Kinetic Isotope Effects

<p>1. Measurement of enzyme binding isotope effects<br>Vern Schramm<br>2. Chemical ligation and isotope labeling to locate dynamic effects<br>Rudolf Alleman<br>3. Measurement of heavy enzyme isotope effects<br>Amnon Kohen<br>4. Extracting kinetic isotope effects from a global analysis of reaction progress curves<br>Sam Hay<br>5. KIE of metabolic flux and enzymes<br>Guillaume Tcherkez<br>6. Solvent and Primary KIE on Flavin Enzymes<br>Paul Fitzpatrick<br>7. The Rapid Determination of Primary Deuterium Isotope Effects on Enzyme-Catalyzed Proton Transfer at Carbon in 50/50 HOH/DOD<br>John Richard<br>8. 18-O isotope effects on oxygenases<br>Judith Klinman<br>9. Chlorine isotope effects on dehalogenases<br>Piotr Paneth<br>10. Chlorine isotope effects (w/Paneth)<br>Agnieszka Dybala-Defratyka<br>11. Kinetic Deuterium Isotope Effects in Cytochrome P450 Reactions<br>F. Peter Guengerich<br>12. Use of Isotopes and Isotope Effects for Investigations of Diiron Oxygenase Mechanisms<br>John Lipscomb<br>13. Characterization of substrate, co-substrate, and product isotope effects associated with enzymatic oxygenations using gas- and liquid chromatography/isotope ratio mass spectrometry<br>Thomas Hofstetter<br>14. NMR titrations to determine equilibrium isotope effects<br>Charles Perrin<br>15. NMR to characterize the nonstatistical distribution of stable isotopes in metabolism<br>Richard Robins<br>16. Applications of isotope ratio mass spectrometry in sports drug testing: accounting for isotope fractionation in analysis of biological samples<br>Thomas Piper<br>17. Whole molecule mass spectrometry to measure KIEs on RNA 2’O-transphosphorylation reactions<br>Mike Harris<br>18. Isotope Effects on the Concentration of Steady State Intermediates<br>Hung-Wen Liu and Mark Ruszczycky <br>19. Headspace analysis of CO2 to measure carbon kinetic isotope effects in decarboxylation reactions<br>Ron Kluger<br>20. 32P/33P remote label methods to measure RNA kinetic isotope effects<br>Joseph Piccirilli<br>21. Isotope effects and MS analysis of pseudouridine synthase enzymes<br>Eugene Mueller<br>22. Nucleophile isotope effects on glycosyl transfer reactions<br>Andrew James Bennet</p>