RNA Modification Enzymes

<p>Preface<br>Jane E. Jackman<br>1. Locating chemical modifications in RNA sequences through ribonucleases and LC-MS based analysis<br>Balasubrahmanyam Addepalli<br>2. Development of RNA modification mapping pipelines for high-throughput sequencing approaches<br>Todd Lowe<br>3. AlkAniline-Seq for high-resolution mapping RNA m7G and m3C modifications<br>Yuri Motorin<br>4. Facile detection of RNA phospho-methylation in cells<br>Blerta Xhemalce<br>5. Detection and analysis of glycosylated queuosine modifications<br>Tao Pan<br>6. A comprehensive pipeline for analysis of RNA 3’-end modification<br>Inna Afasizheva<br>7. Analysis of the epitranscriptome with ion-pairing reagent free oligonucleotide mass spectrometry<br>Stefanie Kellner<br>8. Direct sequencing (ONT) to detect m6A modification<br>9. RNA immunoprecipitation to identify in vivo targets of RNA editing and modifying enzymes<br>Heather A. Hundley<br>10. AdoMet-based labeling of RNA<br>Andrea Rentmeister<br>11. Coupling crosslinking and RNA modification sequencing for identifying RNA modification interacting proteins<br>Juan Alfonzo<br>12. Characterization of codon-specific translation defects using ribosome profiling<br>Sebastian A. Leidel<br>13. Partially modified tRNAs for the study of tRNA maturation and function<br>Ute Kothe<br>14. In vitro assays for complex multi-step RNA modification reactions<br>Dirk Iwata-Reuyl<br>15. Transient kinetic analysis for studying RNA modification enzyme mechanisms<br>Jane E. Jackman<br>16. High-throughput detection and analysis of pseudouridine synthase activities<br>Wendy Gilbert<br>17. Activity assays for wobble inosine modification in tRNA<br>Dragony Fu<br>18. Targeting RNA with site-directed RNA Editing<br>Josh Rosenthal<br>19. RNA pull-down assays for distinguishing tRNA modification substrate specificities<br>Eric Phizicky<br>20. Assessing the consequences of mRNA modifications on protein synthesis using in vitro translation assays<br>Kristin Koutmou<br>21. Mass spectrometry based methods for studying mRNA 5’-cap modifications<br>Tsutomu Suzuki<br>22. CLIP-Seq to identify targets and interactions of RNA modification enzymes<br>Fange Liu<br>23. CRISPR-mediated genome editing targeting RNA modification enzymes<br>Douglas Bernstein</p>