<p>1. High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR T-cell therapy<br>Renpeng Ding, Cheng-Chi Chao and Qianqian Gao<br>2. Determination of the biodistribution of chimeric antigen receptor-modified T cells against CD19 in NSG mice <br>Hairuo Wen, Ying Huang, Tiantian Hou, Junzhi Wang and Yan Huo<br>3. Generation of CAR-T cells using lentiviral vectors<br>Mansour Poorebrahim, Isaac Quiros-Fernandez, Elham Fakhr and Angel Cid-Arregui<br>4. Phenotyping of CAR T cells<br>Sanjay Rathod<br>5. Evaluation of CAR-T cell cytotoxicity: Real-time impedance-based analysis<br>Amanda N. Lisby, Robert D. Carlson, Trevor R. Baybutt, Megan Weindorfer and Adam E. Snook<br>6. Flow cytometry detection and quantification of CAR T cells into solid tumors<br>Nicholas J.A. Tokarew, Javier Suárez Gosálvez, Alessia Nottebrock, Daria Briukhovestka, Stefan Endres, Bruno L. Cadilha and Sebastian Kobold<br>7. Generation and functional characterization of CAR exosomes<br>Chuqi Wang, Wenyan Fu, Changhai Lei and Shi Hu<br>8. Preclinical testing of CAR T cells in zebrafish xenografts<br>Sarah Grissenberger, Benjamin Salzer, Susana Pascoal, Andrea Wenninger-Weinzierl, Manfred Lehner and Martin Distel<br>9. In vivo experimental mouse model to test CD19 CAR T cells generated with different methods<br>Anne Fåne, Marit Renée Myhre, Else Marit Inderberg and Sébastien Wälchli<br>10. Non-invasive fluorescence imaging for tracking immune cells in preclinical models of immunotherapy<br>Marcelo de Souza Fernandes Pereira, Aarohi Thakkar and Dean Anthony Lee<br>11. Generation of CAR T-cells using γ-retroviral vector<br>Norihiro Watanabe and Mary Kathryn McKenna<br>12. Methods to monitor in vivo expansion and efficacy of CAR-T cells in preclinical models<br>Mohammad Alhomoud, Jeremie Martinet, Mayumi Sugita, Alexandra Gomez-Arteaga and Monica L. Guzman<br>13. Strategies for manufacturing cell therapy products aligned with patient needs<br>Prajna Guha and Steven C. Katz</p>