1. RNA and the cellular biochemistry revisited <br>2. Creating a ribonuclease-free environment 000<br>3. Stringency: conditions that influence nucleic acid structure<br>4. Ribonucleic acid isolation strategies<br>5. Isolation of polyadenylated RNA<br>6. The truth about tissues<br>7. Going green: RNA and the molecular biology of plants<br>8. Quality control for RNA preparations<br>9. cDNA: a permanent biochemical record of the cell<br>10. RT-PCR: a science and an art form<br>11. Quantitative polymerase chain reaction techniques<br>12. miRNA and other noncoding RNAs<br>13. RNA interference and gene editing<br>14. Electrophoresis of RNA<br>15. Photodocumentation and image analysis<br>16. Northern analysis<br>17. Nucleic acid probe technology<br>18. Quantification of specific mRNAs by nuclease protection<br>19. Analysis of nuclear RNA<br>20. RNA in situ hybridization<br>21. Array analysis of gene expression<br>22. Subtractive and nonsubtractive methods for the analysis of gene expression<br>23. Transcriptomes and bioinformatics<br>24. RNA-seq: the premier transcriptomics tool<br>25. RNA biomarker discovery and validation<br>26. Functional genomics strategies<br>27. A few RNA success stories <br>Epilogue<br>Appendix A: Maintaining complete and accurate records <br>Appendix B: Converting mass to moles <br>Appendix C: Removal of DNA from an RNA sample <br>Appendix D: Removal of RNA from a DNA sample <br>Appendix E: Electrophoresis: principles, parameters, and safety <br>Appendix F: Disposal of ethidium bromide and SYBR Green solutions <br>Appendix G: Deionization of formamide, formaldehyde, and glyoxal <br>Appendix H: Silanizing centrifuge tubes and glassware <br>Appendix I: Centrifugation as a mainstream tool for the molecular biologist <br>Appendix J: Dot blot analysis <br>Appendix K: Useful stock solutions for the molecular biologist <br>Appendix L: Genomes and proteomes <br>Appendix M: Common SI prefixes <br>Appendix N: Common abbreviations <br>Appendix O: Select suppliers of equipment, reagents, and services <br>Appendix P: Trademark citations <br>Glossary<br>
