<p>1. Simplified acid extraction and quantification of histones in human tumor cells </p><p>Lourdes Hontecillas-Prieto, Daniel J. García-Domínguez, Rocío Flores-Campos; Juan Antonio Flores, Antonio Pérez-Pérez, Luis de la Cruz-Merino, Víctor Sánchez-Margalet and Nabil Hajji </p> <p>2. Expression, purification and characterization of phosphatidylserine-targeting antibodies for biochemical and therapeutic applications </p><p>Varsha Gadiyar, David Calianese, Rachael Pulica, Christopher Varsanyi, Ziren Wang, Ahmed Aquib, Alok Choudhary and Raymond B. Birge </p> <p>3. In vitro ILC differentiation from human HSCs </p><p>Silvia Santopolo, Cecilia Ciancaglini, Francesca Romana Mariotti, Lorenzo Moretta and Linda Quatrini </p> <p>4. Optimizing protocols for human regulatory T cell isolation, expansion and functional characterization </p><p>Sánchez-Moreno Inés, Martín-Otal Celia, Lasarte Juan José and Lozano Teresa </p> <p>5. (1)Specific selection of stimulation-responsive T cells utilizing a short-term activation assay </p><p>Christian Peters, Jara Simeonov, Daniel Gombert and Dieter Kabelitz </p> <p>6. Generation of human and murine exhausted CD8+ T cells in vitro </p><p>Rosa Ana Lacalle, Raquel Blanco, Rebeca García-Lucena and Santos Mañes </p> <p>7. Ex vivo assessment of human neutrophil motility and migration </p><p>Noor A.M. Bakker, Claudia Burrello and Karin E. de Visser </p> <p>8. Flow cytometry-based monitoring of myeloid-derived suppressor cells in the peripheral blood of patients with solid tumors </p><p>Anna-Jasmina Donaubauer, Ilka Scheer, Rainer Fietkau, Udo S. Gaipl, Benjamin Frey </p> <p>9. Deciphering neutrophil heterogeneity in human blood and tumors: Methods for isolating neutrophils and assessing their effect on T-cell proliferation </p><p>Nicolas Delhez, Frank Aboubakar Nana, Camille Houbion, Alexandre Bayard, Annika Bruger, Christophe Vanhaver, Sven Brandau, Pierre van der Bruggen and Thibault Hirsch </p> <p>10. Splenocytes antitumor cytotoxicity assessment after prophylactic vaccination or drug treatment of tumor-bearing mice </p><p>Kenny Misael Calvillo-Rodriguez, Ana Luisa Rivera-Lazarin, Reyes Tamez-Guerra, Ana Carolina Martinez-Torres and Cristina Rodriguez-Padilla </p> <p>11. Therapeutic treatment of tumor-bearing mice with drug-killed cancer cells: A method to confirm immunogenic cell death and assess its therapeutic effectiveness </p><p>Kenny Misael Calvillo-Rodriguez, Maria Norma Gonzalez-Flores, Reyes Tamez-Guerra,Cristina Rodriguez-Padilla, Marilena Antunes-Ricardo and Ana Carolina Martinez-Torres </p> <p>12. Single-cell RNA flow cytometry to assess intratumoral production of cytokines/chemokines </p><p>Khiem C. Lam and Romina S. Goldszmid </p> <p>13. Multidimensional profiling of cancer microenvironments in FFPE tissues by imaging mass cytometry </p><p>Marieke E. Ijsselsteijn and Noel FCC de Miranda </p> <p>14. Evaluation of lymphocyte infiltration into cancer spheroids by immunofluorescent staining and 3D imaging </p><p>Mireia Cruz De los Santos and Andreas Lundqvist </p> <p>15. Quantitative pre-clinical imaging of hypoxia and vascularity using MRI and PET Georgia Kanli, Selma Boudissa, Radovan Jirik, Tom Adamsen, Heidi Espedal, Hans-Olav Rolfsnes, Frits Alan Thorsen, Jesus Pacheco Torres, Bassam Janji and Olivier Keunen </p> <p>16. Retrovirus-based manufacturing of chimeric antigen receptor-modified T cells for cancer therapy research </p><p>Sophia Stock, Luisa Fertig, Vivien D. Menkhoff, Thaddäus Strzalkowski, Manuel Caruso and Sebastian Kobold </p>
