Transketolases

Specificaties
Gebonden, blz. | Engels
Elsevier Science | 2025
ISBN13: 9780443430510
Rubricering
Elsevier Science e druk, 2025 9780443430510
Onderdeel van serie Methods in Enzymology
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Samenvatting

Transketolase is an ubiquitous enzyme which has not yet finished revealing all its secrets. This Methods in Enzymology: Transketolases volume is devoted to the description of cutting-edge methods to its study in biochemistry. Several topics are addressed by world experts in their field.

The first part of this volume deals with transketolase at the cellular level. The latest scientific progress on human transketolase TKL1 in relation to certain diseases and to its inhibition are discussed including the involvement of this enzyme in new metabolic pathways or as a diagnostic biomarker.

The second part focuses on transketolase at the molecular level. The structural and mechanistic aspects linked to this enzyme are addressed using several approaches including molecular modelling and crystallography, for example. Finally, the experimental methodological approaches relating to the use of transketolase as a tool of choice in biocatalysis is presented.

Specificaties

ISBN13:9780443430510
Taal:Engels
Bindwijze:Gebonden

Inhoudsopgave

1. Role of transketolase-like 1 in human cortical neurogenesis<br>Anneline Pinson and Wieland B. Huttner<br>2. Transketolase in metabolic diseases, autoimmune diseases and cancer<br>Xuemei Tong<br>3. Transketolase-like-1 (TKTL1) based one-substrate reaction and its impact on pathologies and treatments<br>Johannes Coy<br>4. Evaluation of binding, affinity and metabolic regulatory activity of a transketolase inhibitor<br>Xiaofei Chen<br>5. Discovery of novel transketolase epitopes and development of IgG-based tuberculosis serodiagnostics<br>Lobelia Samavati<br>6. Transketolases in apicomplexan parasites<br>Nishith Gupta<br>7. Electrochemical screening for microbial transketolase inhibitors identification<br>Chloé Aymard, Bastien Doumèche, Florian Bianco, Jean-François Chateaux, Franck Charmantray and Rainier-Numa Georges<br>8. Transketolases isolation from plants: Diversity of pathways<br>Dorothea Bartels<br>9. Transketolase-sulfoglycolytic pathway<br>Yan Zhang <br>10. Split-gene transketolase homolog that catalyzes the key step of biosynthetic pathway of phosphonothrixin in Saccharothrix sp.<br>Tomohisa Kuzuyama and Yunxun Zhu<br>11. Biophysical and structural analysis of transketolases<br>Kai Tittman and Fabian Rabe von Pappenheim<br>12. Structural variations of transketolases over the evolution from bacteria to higher eukaryotes: in silico/in vitro study<br>Rainier-Numa Georges, Bastien Doumèche, Lionel Ballut and Franck Charmantray<br>13. Structural and biochemical studies on split-gene transketolases, evolutionary aspects and comparisons<br>Jennifer Ann Littlechild<br>14. Transketolase catalytic reaction pathway. Study of ThDP activation and complete catalytic cycle<br>Vincent Thery and Lionel Nauton<br>15. Transketolases: Theoretical aspects from QM and QM/MM investigations<br>Tiziana Marino, Mario Prejanò and Nino Russo<br>16. Computational and structural insights into transketolase function and evolution: A detailed methodology<br>Thommas Mutemi Musyoka and Rita Afriyie Boateng<br>17. Transketolase enzyme discovery and the enzyme evolution of transketolase<br>Alessia Tonoli, Helen C. Hailes, Paul Dalby and Jack Jeffries<br>18. Evolved thermostable transketolase from Geobacillus stearothermophilus for deoxyketoses synthesis<br>Camille Gadona, Franck Charmantray, Laurence Hecquet, Giuseppe Arbia and Muriel Joly<br>19. Practical screening assays to identify the substrate scope of transketolase variants<br>Wolf-Dieter Fessner and Jules Perrard<br>20. Production of rare sugars by transketolase in combination with microbes and other enzymes<br>Akihide Yoshihara, Yoshida Hiromi, Ken Izumori and Shigehiro Kamitori<br>21. Engineered transketolase for cascade enzymatic synthesis with w-transaminase<br>Shuangjun Lin<br>22. Process considerations for the scale-up and implementation of transketolases<br>John Woodley
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