Mark A. Herbert,
Derek W. Hood,
E. Richard Moxon
Humana Press
2003e druk, 2002
9780896039285
Haemophilus influenzae Protocols
Specificaties
Gebonden, 333 blz.
|
Engels
Humana Press |
2003e druk, 2002
ISBN13: 9780896039285
Rubricering
Onderdeel van serie
Methods in Molecular Medicine
Levertijd ongeveer 9 werkdagen
Gratis verzonden
Samenvatting
In HaemophilusiInfluenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible format the major molecular and immunological techniques for exploring the pathogenicity of H. influenzae. Described with step-by-instructions to ensure robust and successful experimental results, the techniques cover plasmid analysis, proteomics, genomics, DNA array technology, gene expression, mutagenesis (transposon and nontransposon), and structural analysis. These methods illuminate how the bacterium causes disease, as well as how best to develop novel vaccines and antibiotics against the organism.
Specificaties
ISBN13:9780896039285
Taal:Engels
Bindwijze:gebonden
Aantal pagina's:333
Uitgever:Humana Press
Druk:2003
Inhoudsopgave
The Pathogenesis of Disease Due to Nontypeable Haemophilus influenzae.- The Pathogenesis of Disease Due to Type b Haemophilus influenzae.- General Methods for Culturing Haemophilus influenzae.- Transformation of Haemophilus influenzae.- Diagnosis of Infection.- Characterization of Plasmids.- Protein Characterization by Two-Dimensional Gel Electrophoresis.- Monitoring Gene Expression Using DNA Arrays.- Gene Expression Technology.- The Genome Sequence of Haemophilus influenzae.- Structural Profiling of Short-Chain Lipopolysaccharides from Haemophilus influenzae.- Mutagenesis of H. influenzae.- Transposon Tn 10.- In Vivo Expression of Bacterial Genes During Human Infections.- ELISA.- Opsonophagocytosis Assay Using Flow-Cytometry.- In Vitro Models of Infection I—Human Respiratory Tissue Organ Culture.- In Vitro Models of Infection II—Human Umbilical Vein Endothelial Cells (HUVECs) System.- Animal Models.