Handbook of ELISPOT

<div>Challenges in Developing Protein Secretion Assays at a Single-Cell Level.-&nbsp;To Count of Not to Count: ELISPOT Evaluation Revisited.-&nbsp;Essential Controls for ELISpot Assay.-&nbsp;Automatic Search of Spots and Color Classification in ELISPOT Assay.-&nbsp;Four Color ImmunoSpot ® Assays for Identification of Effector T Cell Lineages.-&nbsp;Detection of Cross-Reactive B Cells Using the FluoroSpot Assay.-&nbsp;Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations.-&nbsp;Detecting All Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assays.-&nbsp;Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot® Software Based on Center of Mass Distance Algorithm.-&nbsp;Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X™ Suite.-&nbsp;B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells.-&nbsp;Identification of Novel Mycobacterial Targets for Murine CD4+T Cells by IFN ELISPOT.-&nbsp;ELISPOT-Based “Multi-Color FluoroSpot” to Study Type-Specific and Cross-Reactive Responses in Memory B Cells after Dengue and Zika Virus Infections.-&nbsp;Cultured ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses.- Ex Vivo ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses.-&nbsp;Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue Infection.-&nbsp;Dendritic Cell-Based ELISpot Assay for Assessing T Cell-IFN- γ Responses in Human Peripheral Blood Mononuclear Cells to Dengue Envelope Proteins.-&nbsp;Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccines.-&nbsp;Detection and Quantification of Influenza A/H1N1 Virus-Specific Memory B Cells in Human PBMCs using ELISpot Assay.</div><div><br></div>