T-Cell Motility

Methods and Protocols

Specificaties
Gebonden, blz. | Engels
Springer New York | 2019
ISBN13: 9781493990351
Rubricering
Springer New York e druk, 2019 9781493990351
Onderdeel van serie Methods in Molecular Biology
€ 122,99
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Samenvatting

This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells; GapmeR-mediated gene silencing in motile T-cells; activity of cellular kinases in migrating T-cells; and computational analysis of protein-protein interactions in motile T-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, T-Cell Motility: Methods and Protocols is an essential resource for graduate students, postdoctoral fellows, and principal investigators working in the fields ofimmunology, T-cell biology, biochemistry, molecular biology, and imaging.

Specificaties

ISBN13:9781493990351
Taal:Engels
Bindwijze:gebonden
Uitgever:Springer New York

Inhoudsopgave

<div>An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility.-&nbsp;Isolation of Human Peripheral Blood T Lymphocytes.-&nbsp;A Laboratory Model to Study T-Cell Motility.-&nbsp;Quantification of T-Cell Migratory Phenotypes using High Content Analysis.-&nbsp;Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time.-&nbsp;Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signalling Cross-Talks in Motile T-Cells.-&nbsp;Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis.-&nbsp;Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes.-&nbsp;GapmeR-Mediated Gene Silencing in Motile T-Cells.-&nbsp;Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices using Confocal Microscopy.-&nbsp;Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes.-&nbsp;Immunometabolomic Phenotyping of Motile T-Cells.-&nbsp;Profiling Activity of Cellular Kinases in Migrating T-Cells.-&nbsp;Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes.-&nbsp;A Protocol to Study T-Cell Signalling in an Immune Synapse by Microscopy.-&nbsp;Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides.-&nbsp;Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility.-&nbsp;Computational Analysis of Protein-Protein Interactions in Motile T-Cells.</div><div><br></div>
€ 122,99
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        T-Cell Motility