Tumor Microenvironment

1.Staining against phospho-H2AX (γ-H2AX) as a marker for DNA damage and genomic instability in cancer tissues and cells.- 2.Measuring DNA replication rates in hypoxic conditions.- 3.Isolation of Proteins on Nascent DNA in hypoxia and reoxygenation conditions.- 4.In vivo interrogation of the hypoxic transcriptome of solid tumors: optimizing hypoxic probe labelling with Laser-Capture Microdissection for isolation of high-quality RNA for deep sequencing analysis.- 5.Gas chromatography coupled to mass spectrometry (GC-MS) to study metabolism in cultured cells.- 6.Probing Cancer Cell Metabolism using NMR Spectroscopy.- 7.Measuring the Impact of Microenvironmental Conditions on Mitochondrial Dehydrogenases Activity in Cultured Cells.- 8.Measuring Autophagy in the Context of Cancer.- 9.Methods for studying autophagy within the tumor microenvironment.- 10.Using 3 Dimensional Culture (Spheroids) as an in vitro Model of Tumour Hypoxia.- 11.Isolation of Glioma Initiating Cells for Biological Study.- 12.Isolation of mouse and human tumor associated macrophages.- 13.Mouse models of brain metastasis for unravelling tumour progression.- 14.Quantification of lung metastases from in vivo mouse models.- 15.A multimodal data analysis approach for targeted drug discovery involving topological data analysis (TDA).- 16.Efficient protocol for the identification of hypoxic cell radiosensitisers.