Section 1 - Carbohydrate-Protein Interactions-Physical Measurements <br><br>Characterization of specific donor binding to £\1,4-N-acteylhexosaminyltransferase EXTL2 using Isothermal Titration Calorimetry.<br>NMR Analysis of Carbohydrate-Protein Interactions.<br>Determination of atomic structure for glycosyltransferase-substrate complex.<br><br>Section 2 ¡V Phage Display Library<br><br>Screening of peptide-displaying phage libraries to identify short peptides mimicking carbohydrates.<br>Phage Display-Derived Human Antibodies Against Specific Glycosaminoglycan epitopes.<br><br>Section 3 - Glycosyltransferase Expression Profiling<br><br>Autofluorescent proteins for monitoring the intracellular distribution of glycosyltransferases.<br>Expression profiling of glycosyltransferases and their related enzymes using in situ hybridization.<br>Expression profiling of glycosyltransferases using RT-PCR.<br>Expression profiling of lycosyltransferases and related enzymes using gene micro-arrays.<br><br>Section 4 - CHO mutants in Glycan Biosynthesis<br><br>Lectin Resistant CHO Glycosylation Mutants.<br>CHO glycosylation mutants ¡V GPI anchor.<br>CHO glycosylation mutants ¡Vproteoglycans.<br><br>Section 5 - Function of Sulfation<br><br>Determination of Substrate Specificity of Sulfotransferases and Glycosyltransferases (Protoglycans).<br>Measuring the activities of the sulfs, two novel heparin/heparan sulfate endosulfatases.<br>Determination of chemokine-glycosaminoglycan interaction specificity.<br>Identification of Proteoglycan-binding Proteins.<br>Functions of glycans revealed by gene inactivation of L-selectin ligand sulfotransferases in mice.<br><br><br>Section 6 - Functions Revealed by Forced Expression or Gene Knockout<br><br>Expression of specific carbohydrates by transfection with specific carbohydrate modifying enzymes.<br>N-glycan structure analysis using lectins and an a-mannosidase IIx activity assay.<br>Targeted disruption of the gene encoding core 1 ƒÒ1-3-galactosyltransferase (T-synthase) causes embryonic lethality and defective angiogenesis in mice. <br>Roles of Mucin-Type O-glycans Synthesized by Core2B1,6-N-aetylglucosaminyltransferase.<br>Analysis of leukocyte rolling in vivo and in vitro.<br>Cell type-dependent Roles of Carbohydrates in Tumor Metastasis.
