<br>Preface</br><br>List of Abbreviations</br><br>Part 1: Introduction</br><br> 1.1 Background</br><br> 1.2 Aim, Scope and Purpose of the Book</br><br> 1.3 Organization of the Book</br><br> 1.4 Terminology</br><br> 1.5 Regulatory Compliance </br><br> 1.6 References</br><br>Part 2: General Considerations</br><br> 2.1 Clinical Information</br><br> 2.2 Basic Principles of Molecular Analysis </br><br> 2.3 Troubleshooting</br><br> 2.4 References </br><br>Part 3: Protocols</br><br> 3.1 Sample Shipment and Storage</br><br> 3.2 Reagents</br><br> 3.3 Harvesting WBCs by RBC Sedimentation </br><br> 3.4 Short-term Cell Cultures from Amniotic Fluid</br><br> 3.5 Cultured Amniocyte Cell Harvesting </br><br> 3.6 Cell Preparation and DNA Extraction</br><br> 3.7 Assessment of DNA Quality, Quantity and Purity</br><br> 3.8 PCR Amplification</br><br> 3.9 Restriction Enzyme Digestion for PCR-RFLP Analysis</br><br> 3.10 Allele-specific PCR</br><br> 3.11 Agarose Gel Preparation and Electrophoresis</br><br> 3.12 Acrylamide Gel Preparation and Electrophoresis</br><br> 3.13 Single-strand Conformation Polymorphism (SSCP)</br><br> 3.14 Optimizing a PCR </br><br> 3.15 Gene Zygosity Assessment by Quantitative PCR Amplification</br><br> 3.16 VNTR and STR Analysis by PCR Amplification</br><br> 3.17 Multiplex PCR Analysis </br><br> 3.18 Southern Analysis </br><br> 3.19 References</br><br>Part 4: Red Cell Blood Groups</br><br> 4.1 Terminology</br><br> 4.2 Clinical Applications</br><br> 4.3 Situations where Genotype and Phenotype do not Agree</br><br> 4.4 References </br><br> 4.5 Facts Sheets, Gene Maps and Molecular Protocols</br><br>Part 5: Platelet Blood Groups</br><br> 5.1 Terminology</br><br> 5.2 Clinical Applications</br><br> 5.3 References </br><br> 5.4 Facts Sheets, Gene Maps and Molecular Protocols</br><br>Part 6: Neutrophil Blood Groups </br><br> 6.1 Terminology</br><br> 6.2 Clinical Applications </br><br> 6.3 References</br><br> 6.4 Facts Sheets, Gene Maps and Molecular Protocols</br><br>Part 7: Appendices</br><br> 7.1 Database Information</br><br> 7.2 Glossary</br><br> 7.3 References </br><br> 7.4 Works Sheets and Request Forms</br><br>Index</br><br>Useful Tables</br>
