Single Molecule Tools, Part A: Fluorescence Based Approaches

1. Watching single DNA replication loops under flow extension<br>2. Star polymer surface passivation for single molecule detection<br>3. Ultrahigh resolution detection of single active motor proteins in live cells<br>4. Molecules and Methods for Superresolution Imaging<br>5. Aqueous nanodroplets for studying single molecules<br>6. High-speed atomic force microscopy techniques for visualizing dynamic behavior of biological macromolecules<br>7. Single-Biomolecule Spectroscopy Using Microfluidic Platforms<br>8. DNA Looping Kinetics Analyzed by Tethered Particle Microscopy<br>9. Single molecule observation of proteins in vivo<br>10. DNA curtains as a high-throughput approach to single molecule imaging<br>11. Single-molecule enzymology of protein synthesis<br>12. Single molecule fluorescence studies of intrinsically disordered proteins<br>13. Nanovesicle trapping for studying transient protein-protein interactions by single molecule FRET<br>13. Tracking single motor proteins in the cytoplasm of mammalian cells<br>14. Conformational States of F1-ATPase by Single-Molecule Rotation <br>15. Single Molecule Sequencing by Fluorescence Imaging<br>16. Real-Time DNA Sequencing from Single Polymerase Molecules<br>17. Micropatterning and single molecule imaging for quantitative analysis of protein-protein interactions in living cells<br>18. Probing virus-receptor interactions by atomic force spectroscopy<br>19. Single-Molecule Fluorescence Spectroscopy of Cytochrome P450 in Nanodiscs<br>20. Analysis of complex single molecule FRET time traces <br>21. Application of super-resolution imaging to single particle tracking in nanotechnology <br>22. Scanning FCS for the characterization of protein dynamics in live cells <br>23. Single mRNA molecule tracking in live cells <br>24. Single-molecule high-resolution colocalization (SHREC) or Single-molecule optical-trap analyses of protein structure<br>25. Nanopore force Spectroscopy tools for analyzing single bio-complexes <br>26. Use of plasmon coupling to reveal DNA dynamics at the single molecule level <br>27. Fluorescence-force spectroscopy<br>28. Multiplexed single mRNA imaging in fixed cells<br>29. Size-Minimized Quantum Dots for Single-Molecule and Intracellular Imaging<br>30. The ABEL trap <br>31. An optical torque wrench <br>32. Determining the Stoichiometry of Protein Hetero-complexes in Living Cells with Fluorescence Fluctuation Spectroscopy <br>33. Fluorescent Visualization of Single Protein-DNA Complexes<br>34. Direct Measurement of Tertiary Contact Cooperativity in RNA Folding by single molecule FRET<br>35. Nanometer-localized multiple single-molecule (NALMS) 36. Multiparameter single molecule fluorescence detection with applications to FRET<br>37. Single-particle tracking-photoactivated localization microscopy (sptPALM) within live cells<br>38. Site-specific incoporation of fluorescent probes into RNA polymerase <br>39.Quantitative single-molecule imaging by confocal laser scanning microscopy <br>40. Studies of DNA-replication at the single molecule level using magnetic tweezers <br>41. RNA labeled for single molecule FRET analysis from ligation with T4 RNA ligases<br>42. Combining optical tweezers, single-molecule fluorescence microscopy and microfluidics for studying reversible protein-DNA interactions <br>43. How dwell time distributions and other such observables in single molecule analysis can be used to extract information from molecular systems