Cell Biology Assays

<ol> <li>Permeabilized Epithelial Cells to Study Exocytic Membrane Transport</li> <li>Studying Excit and Surface Delivery of Post-Golgi Transport Intermediates Using in vitro and Live-Cell Microscopy-Based Approaches</li> <li>Assays Measuring Membrane Transport in the Endocytic Pathway</li> <li>Microsome-Based Assay for Analysis of Endoplasmic Reticulum to Golgi Transport In Mammalian Cells</li> <li>Cotranslational Translocation of Proteins into Microsomes Derived from the Rough Endoplasmic Reticulum of Mammalian Cells</li> <li>Use of Permeabilsed Mast Cells to Analyze Regulated Exocytosis</li> <li>Syringe Loading: A Method for Assessing Plasma Membrane Function as a Reflection of Mechanically Induced Cell Loading</li> <li>Cell Surface Biotinylation and Other Techniques for Determination of Surface Polarity of Epithelial Monolayers</li> <li>Protein Translocation into Mitochondria</li> <li>Polarographic Assays of Mitochrondrial Functions</li> <li>Analysis of Nuclear Protein Import and Export in Digitonin-Permeabilized Cells</li> <li>Heterokaryons: An Assay for Nucleocytoplasmic Shuttling</li> <li>DNA Replication-Dependent Chromatin Assembly System</li> <li>Cygnets: Intracellular Guanosine 3’,5-Cycli Monophosphate Sensing in Primary Cells Using Fluorescence Energy Transfer</li> <li>Ca2+ as a Second Messenger: New Reporters for Calcium (Cameleons and Camgaroos)</li> <li>Ratiometric Pericam</li> <li>Fuorescent Indicators for Imaging Protein Phosphorylation in Single Living Cells</li> <li>In Situ Electroporation of Radioactive Nucleotides: Assessment of Ras Activity or 32P Labeling of Cellular Proteins</li> <li>Dissecting Pathways; in Situ Electroporation for the Study of Signal Transduction and Gap Junctional Communication</li> <li>Detection of Protein-Protein Interactions in vivo Using Cyan and Yellow Fluorescent Proteins</li> <li>Tracking Individual Chromosomes with Integrated Arrays of Iacop Sites and GFP-laci Repressor: Analyzing Position and Dynamics of Chromosomal Loci in Saccharomyces cerevisiae</li> <li>Microtubule Motility Assays </li> <li>In vitro Assays for Mitotic Spindle Assembly and Function</li> <li>In vitro Motility Assays with Actin</li> <li>Use of Brain Cytosolic Extracts for Studying Actin-Based Motility of Listeria monocytogenes</li> <li>Pedestal Formation by Pathogenic Escherichia coli: A Model System for Studying Signal Transduction to the Actin Cytoskeleton</li> <li>Listeria monocytogenes: Techniques to Analyze Bacterial Infection in vitro</li> <li>Measurement of Cellular Contractile Forces Using Patterned Elastomer</li></ol>