Preface. <strong>1: Introduction to Retroviruses and Retroviral Vectors;</strong> G.L. Buchschacher Jr. Introduction. General Background and Classification of Retroviruses. The Retrovirus Genome and Virion Structure. The Retrovirus Replication Cycle. Elements of Retroviral Vector Systems. Summary. Acknowledgements. <strong>2: HIV-1 Replication;</strong> E.O. Freed. Introduction. The HIV-1 Replication Cycle. Role of the HIV Accessory Proteins in Virus Replication. Concluding Remarks. <strong>3: Determinants for</strong> <strong>Lentiviral Infection of Non-Dividing Cells;</strong> M.A. Vodicka. Introduction. Nuclear Transport. Transit of Non-Retroviral Particles. Retroviral Preintegration Complexes. Assays for Infection of Non-Dividing Cells. Viral Protein Determinants for HIV-1 Infection of Non-Dividing Cells. Viral Genome Structural Determinants. Summary and Conclusions. <strong>4: HIV-1 Vector Systems;</strong> N. Srinivasakumar. Introduction. HIV-1 Vector Systems Development. Clinical Applications of HIV-1 Vectors. Anticipated Developments in HIV-1-Based Packaging Systems and Possible Confounding Factors. Conclusions. Acknowledgements. <strong>5: HIV-2</strong> <strong>and SIV Vector Systems</strong>;<strong> </strong>J.R. Gilbert, F. Wong-Staal. Introduction. Classification and Distribution. Pathology and Viral Replication. Genome Organization and Regulation. Vector Systems. Requiem and Prospectus. <strong>6: Vector Systems;</strong> S.L. Sauter, M. Gasmi. Epidemiology and Pathogenesis of FIV Infection. Development of FIV-Based Vectors. Acknowledgements. <strong>7: EIAV, CIAV and Other Lentevirus Vector Systems;</strong> J.C. Olsen. Introduction. Biological Similarities and Differences of Lenteviruses. Recent Vector Developments. <strong>8: Safety Considerations in</strong> <strong>Vector Development.</strong> J.C. Kappes, Xiaoyun Wu. Introduction. Current Safety Design. Advancing Lenteviral Vectors Toward the Clinic. <strong>9:</strong> <strong>Prospects for Gene Therapy Using HIV-Based Vectors</strong>; Jiing-Kuan Yec, J.A. Zaia. Advantages of HIV Vectors Support Development. Attempts to Engineer Biosafety into HIV Vectors. Potential Clinical Applications of HIV Vectors. Regulatory Issues. Acknowledgements. <strong>10: Ethical</strong> <strong>Considerations in the Use of Lenteviral Vectors for Genetic Transfer</strong>; I. Roy. Introduction. Gene Therapy: Potentially Problematic Types of Application. General Ethical Considerations: Research and Clinical Settings. Summary. Index.
