Buddhi Prakash Jain,
Shweta Pandey,
Shyamal K Goswami
Elsevier Science
e druk, 2024
9780443139451
Protocols in Biochemistry and Clinical Biochemistry
Specificaties
Paperback, blz.
|
Engels
Elsevier Science |
2024
ISBN13: 9780443139451
Rubricering
Levertijd ongeveer 9 werkdagen
Gratis verzonden
Samenvatting
Protocols in Biochemistry and Clinical Biochemistry, second edition, offers clear, applied instruction in fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose, and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting, all to support a range of study types and clinical diagnostics.
This fully revised edition has been expanded and enriched to feature 100 protocols, as well as chapter key term definitions and worked examples. All-new protocols added to this edition include identification of lipids by TLC, lipid per oxidation measurement by thiobarbituric acid assays, determination of serum amylase, catalase activity assay, superoxide dismutase assay, qualitative analysis of plant secondary metabolites, qualitative analysis of photochemicals, quantitative estimation of secondary metabolites, estimation of chlorophyll contents, and starch determination, among others. Each protocol is written to help researchers and clinicians easily reproduce lab methods and ensure accurate test results.
Specificaties
Inhoudsopgave
Solutions, Acids and Bases<br>1. Preparation of Solutions<br>2. Acid and Base<br><br>Carbohydrates<br>3. Determination of the presence of carbohydrates in the given sample by Molisch’s test<br>4. Determination of the presence of reducing carbohydrates in the given sample by Picric acid test<br>5. Determination of the presence of reducing carbohydrates in the given sample by Fehling’s test<br>6. Determination of the presence of reducing carbohydrates in the given sample by Benedict’s test<br>7. Determination of the presence of reducing carbohydrates in the given sample by Tommer’s test<br>8. Determination of the presence of reducing carbohydrates in the given sample by Nylander’s test<br>9. Distinguish between monosaccharides and reducing disaccharides by Barfoed’s test.<br>10. Distinguish between aldose and ketose by Seliwanoff’s test.<br>11. Determination of pentose sugar by Bial’s test<br>12. Determination of the presence of galactose in a test sample by the Mucic acid test<br>13. Differentiation between the presence of ketohexose or aldohexose in the test sample by Foulger'stest<br>14. Determination of the presence of reducing carbohydrates in the given sample by Fearon’s methylamine test<br>15. Confirmation of the presence of reducing carbohydrates in the given sample by osazone formation test<br>16. Determination of the presence of polysaccharides in the given sample by an iodine test<br>17. Quantitative estimation of carbohydrates in the given sample by anthrone test.<br>18. Quantitative estimation of reducing carbohydrates in the given sample by 3,5-dinitro salicylic acid (DNSA) test.<br>19. Extraction and estimation of glycogen from the liver and muscle of the well-fed and starved rat.<br>20. Separation and Identification of sugars present in fruit juices using thin-layer chromatography (TLC).<br>21. Extraction and analysis of soluble carbohydrates from plants.<br><br>Lipids<br>22. Solubility test for lipids<br>23. Emulsion test for lipids.<br>24. To determine the degree of unsaturation of fatty acids<br>25. Determination of the saponification value of the given fat/ oil sample<br>26. To determine the fatty acid value of fat<br>27. Acrolein test for the presence of glycerol<br>28. Libermann – Buchard test for detection of cholesterol.<br>29. Extraction of lipid from leaves (Bligh and Dyer’s method and single extraction method).<br>30. Extraction of lipid from egg yolk and estimation of phosphorous content in it<br>31. Extraction of lipid from tissues (Folch method).<br>32. Identification of lipids by TLC.<br>33. Lipid per oxidation measurement by Thiobarbituric acid (TBA) assay.<br><br>Proteins<br>34. Isolation of protein from animal cells/tissue using RIPA buffer<br>35. Qualitative test for Proteins and amino acids<br>36. Estimation of proteins by Biuret method<br>37. Estimation of proteins by Bradford method<br>38. Estimation of proteins by Bicinchoninic acid (BCA) method<br>39. Estimation of proteins by Folin-Lowry method<br>40. Estimation of proteins by UV spectrophotometric method<br>41. Protein separation by gel electrophoresis under denaturing condition (SDS-PAGE)<br>42. Separation of amino acids by paper chromatography.<br><br>Minerals<br>43. Estimation of the Calcium in water or milk by EDTA titrimetric method<br>44. Estimation of the Calcium in serum by O-Cresolpthaleincomplexon method (colorimetric)<br>45. Estimation of the inorganic phosphorus by colorimetric method<br>46. Estimation of the Iron by thiocyanate colorimetric method<br>47. Estimation of the Chloride by Titration method (Mohr Argentometric method)<br><br>Vitamin<br>48. Estimation of Vitamin A by Trichloroacetic acid (modified Carr-Price method)<br>49. Estimation of the Tocopherol (Vitamin E) by colorimeter (Baker and Frank method)<br>50. Estimation of the Thiamine (Vitamin B1) by Thiochrome technique<br>51. Estimation of the Ascorbic acid (Vitamin C) by 2,6-dichlorophenol indophenol (DCPIP) method (Titrimetric)<br>52. Estimation of the Ascorbic acid (Vitamin C) by 2, 4 Dinitrophenylhydrazine method (Colorimetric)<br><br>Enzymes<br>53. To study the effect of pH and temperature on the action of salivary amylase.<br>54. Determination of the activity of amylase enzyme (crude) extracted from sweet potato.<br>55. Determination of the specific activity of the amylase enzyme.<br>56. To determine Vmax and Km in of the amylase enzyme.<br><br>Nucleic acid<br>57. Determination of the amount of DNA present in the solution by diphenylamine (DPA) method<br>58. Determination of the amount of RNA present in the solution by orcinol method.<br>59. Isolation of plasmid DNA using the alkaline lysis method.<br>60. Preparation of plasmid DNA using boiling lysis method.<br>61. Plasmid DNA purification using polyethylene glycol.<br>62. Isolation of genomic DNA of the unknown bacterial strain.<br>63. Isolation of DNA from plant tissue.<br>64. Isolation of DNA from a eukaryotic cell using proteinase K and phenol.<br>65. Isolation of DNA from a eukaryotic cell.<br>66. Separation of bands of DNA by agarose gel electrophoresis.<br>67. SOUTHERN TRANSFER AND HYBRIDIZATION: To perform Southern blotting of the genomic DNA.<br>68. Isolation of RNA from animal cells.<br>69. NORTHERN BLOTTING.<br><br>Clinical Biochemistry<br>70. Determination of Serum Urea [Diacetyl Monoxime (DAM) Method],<br>71. Estimation of Uric Acid in Serum Sample (Uricase Method),<br>72. Estimation of Creatinine in Serum Samples (Jaffe’s Method),<br>73. Determination of Serum Glutamate Pyruvate Transaminase (SGPT) Activity,<br>74. Determination of Serum Glutamate Oxaloacetate Transaminase (SGOT) Activity,<br>75. Colorimetric Estimation of Alkaline Phosphatase (ALP) From Serum (King and Armstrong),<br>76. Estimation of Serum Albumin by the Dye-Binding Method (Bromocresol Green Method),<br>77. Estimation of Blood Glucose by the Glucose Oxidase Method,<br>78. Estimation of Glucose in Urine by Benedict Method,<br>79. Estimation of Percentage of Glucose (Reducing Sugars) in Urine (by Benedict Titration Method),<br>80. Oral Glucose Tolerant Test (OGTT),<br>81. Estimation of Total Cholesterol in Serum (Watson Method),<br>82. Estimation of Total Cholesterol in Serum (Enzymatic Method),<br>83. Estimation of Triglycerides in Serum (Enzymatic Method),<br>84. Estimation of Hemoglobin in the Blood (Sahli’s Method).<br>85. Determination of serum amylase.<br>86. Catalase activity assay.<br>87. Superoxide dismutase (SOD) assay.<br><br>Plant Biochemistry<br>88. Qualitative analysis of plant secondary metabolites.<br>89. Qualitative analysis of photochemical.<br>90. Quantitative estimation of secondary metabolites.<br>91. Estimation of Chlorophyll contents.<br>92. Determination of starch