New Experimental Probes for Enzyme Specificity and Mechanism

1. Subverting Hedgehog Protein Autoprocessing by Chemical Induction of Paracatalysis<br>2. New Mechanistic Probes to Identify Novel Substrates for N-Myristoyltransferases<br>3. Phosphonate and α Fluorophosphonate Analogues of D Glucose 6 Phosphate as Active-Site Probes of 1L-Myo-Inositol 1 phosphate Synthase<br>4. Kinetic Mechanism of Nicotine-Degrading Enzyme Probed by Stopped-Flow Kinetic Analyses<br>5. Kinetics and Mechanism for Enzyme-Catalyzed Reactions of Substrate Pieces<br>6. Kinetics and Mechanism for Reactions of Enzyme Pieces<br>7. Evaluation of allostery for the bienzyme assembly of a 3-deoxy-D-arabino heptulosonate-7-phosphate synthase and chorismate mutase<br>8. Recognition and Catalysis of Reactions of Chiral Substrates by Mandelate Racemase<br>9. Innovative and emerging modalities of EGFR kinase inhibitors<br>10. Characterization of the Aminoacrylate Intermediate of Tyrosine Phenol-Lyases<br>11. Employing deuterium kinetic isotope effects to uncover the mechanism of (R)-3-hydroxybutyrate dehydrogenase<br>12. Probing the mechanism of flavin action in the oxidative decarboxylation catalyzed by salicylate hydroxylase<br>13. Kinetic Characterization of Methylthio-D-ribose-1-phosphate Isomerase<br>14. The Role of Protein Motions in Catalysis<br>15. The nickel-pincer coenzyme of lactate racemase: A case study of uncovering cofactor structure and biosynthesis<br>16. Adaptation of Transient State Methods to Reveal the Mechanism of Mammalian Dihydropyrimidine Dehydrogenase<br>17. Charge Resonance in Enzyme Redox Reactions<br>18. Motions and Allostery in Human Glucokinase<br>19. Kinetic Dissection of CRISPR CAS-9<br>20. Oxygenase PLP Chemistry<br>21. Electron bifurcating flavoproteins