Biophysical Approaches for the Study of Membrane Structure Part A

Preface<br>Tobias Baumgart and Markus Deserno<br>1. Evaluation of functional transbilayer coupling in live cells by controlled lipid exchange and imaging FCS<br>Nirmalya Bag, Arpita Tripathy and Sudipti Priyadarsinee<br>2. Fluorescence imaging of lamellipodin mediated biomolecular condensates on solid supported lipid bilayer membranes<br>Tobias Baumgart<br>3. Effects of lateral and hydrostatic pressure on membrane structure and properties<br>Nick Brooks<br>4. Using the yeast vacuole as a system to test the lipid drivers of membrane heterogeneity in living cells<br>Itay Budin, Israel Juarez-Contreras and Hyesoo Kim<br>5. The spectral phasor approach to resolving membrane order with environmentally sensitive dyes<br>Rumiana Dimova and Agustín Mangiarotti<br>6. The use of hemifusion to create asymmetric giant unilamellar vesicles: Insights on induced order domains<br>Thais A. Enoki<br>7. Advanced microscopy methods to study membrane pores<br>Ana J. García-Sáez<br>8. Use of cryo-EM to study membrane phase separation<br>Frederick Heberle and M. Neal Waxham<br>9. Using lipid binding proteins and advanced microscopy to study lipid domains<br>TOSHIHIDE KOBAYASHI, NARIO TOMISHIGE, KOHTA TAKAHASHI, BRIGITTE POLLET, LUDOVIC RICHERT and YVES MÉLY<br>10. Structural characterization of lateral phase separation in polymer-lipid hybrid membranes<br>Cecília Leal, Nurila Kambar, Minh N. Do, Corey Snyder and Yoo Kyung Go<br>11. Applications of phase-separating multi-bilayers in protein-membrane domain interactions<br>Kandice R. Levental and Hong-Yin Wang<br>12. Studying lipid flip-flop using NMR and TR-SANS<br>Drew Marquardt, Dominik Dziura and Maksymilian Dziura<br>13. Thickness determination of hydroperoxidized lipid bilayers from medium-resolution cryo-TEM images<br>Carlos Marques, André Schroder, Marc Schmutz, Pierre Muller and Eulalie J. Lafarge<br>14. Structure of symmetric and asymmetric lipid membranes from joint SAXS/SANS<br>Georg Pabst, Enrico Semeraro and Moritz Frewein<br>15. Quantification of membrane geometry and protein sorting on cell membrane protrusions using fluorescence microscopy<br>Zheng Shi and Shilong Yang<br>16. A TIRF microscopy-based method for observing the uptake of individual particles by clathrin mediated endocytosis<br>Jeanne Stachowiak, Grant Ashby, Kayla Keng and Carl Hayden<br>17. Pore-spanning membranes as a tool to investigate lateral lipid membrane heterogeneity<br>Claudia Steinem and Larissa Socrier<br>18. MαCD based plasma membrane outer leaflet lipid exchange in mammalian cells to study insulin receptor activity<br>Pavana Suresh and Erwin London