<p>1. Flow cytometry analysis of myeloid derived suppressor cells using 6 color labeling </p><p>Rocío Flores-Campos, Daniel J. García-Domínguez, Lourdes Hontecillas-Prieto, Carlos Jiménez-Cortegana, Luis de la Cruz-Merino and Víctor Sánchez-Margalet </p> <p>2. In vitro screening methods of novel immune checkpoint inhibitors related to T cell infiltration and anti-PD-1 resistance </p><p>Zhuoying He, Xiuman Zhou, Youmei Xiao and Yanfeng Gao </p> <p>3. Machine learning approach to assess brain metastatic burden in preclinical models. </p><p>Jessica Rappaport, Quanyi Chen, Tomi McGuire, Amélie Daugherty-Lopès and Romina Goldszmid </p> <p>4. Cytofluorometric analysis of the maturation and activation of bone marrow-derived dendritic cells to assess immunogenic cell death </p><p>Faye Naessens, Iuliia Efimova, Mariia Saviuk and Dmitri V. Krysko </p> <p>5. Functional evaluation of circulating anti-cancer antibodies with a 3D tumor cell growth inhibition assay </p><p>Francesca Ruzzi, Maria Sofia Semprini, Laura Scalambra, Stefania Angelicola, Chiara Cappello, Olga Maria Pittino, Patrizia Nanni, Arianna Palladini and Pier-Luigi Lollini </p> <p>6. Spatial immunophenotyping of FFPE tissues by imaging mass cytometry </p><p>Carolina Mangana and Barbara B. Maier </p> <p>7. Processing human colon cancer specimens for in vitro cytotoxicity assays </p><p>Sofia Mensurado, Rafael Blanco-Domínguez, Mariana Carreira, Leandro Barros and Bruno Silva-Santos </p> <p>8. An orthotopic metastatic xenograft model of colorectal cancer </p><p>Rafael Blanco-Domínguez, Sofia Mensurado, Leandro Barros, Mariana Carreira and Bruno Silva-Santos </p> <p>9. A preclinical mouse model of hepatic metastasis to instruct effective treatment modalities </p><p>Arianna Pocaterra, Antonio Citro, Chiara Gnasso, Tamara Canu, Anna Tosi, Antonio Rosato, Antonio Esposito, Lorenzo Piemonti and Anna Mondino </p> <p>10. Myeloid-derived suppressor cells: Identification and function </p><p>Paola Vacca, Maria Teresa Bilotta, Lorenzo Moretta and Nicola Tumino </p> <p>11. Measuring the impact of therapy-induced senescence on NK cell phenotypes in cancer </p><p>Shreya R. Chowdhury, Katherine C. Murphy, Chaitanya N. Parikh, Kelly D. DeMarco, Lin Zhou and Marcus Ruscetti </p> <p>12. Selective expansion of anti-tumor innate lymphocytes in long-term cultures after a single BCG pulse </p><p>María-José Felgueres, Gloria Esteso, Nacho Aguiló and Mar Valés-Gómez </p>