Preface<br>Section I IMMUNOASSAY TECHNOLOGY<br>RIA (Radioimmunoassay)<br> 1. Yalow, R.S., Berson, S.A. (1960: Assay of plasma insulin in man.<br>FPIA (Fluorescence Polarization Immunoassay)<br> 2. Dandliker, W.B., Feigen, G.A. (1961): Quantification of the antigen-antibody reaction by the polarization of fluorescence. <br>CPB (Competitive Protein Binding)<br> 3. Murphy, B.E.P., Pattee, C.J. (1964): Determination of thyroxine utilizing the property of protein-binding. <br>ELISA (Enzyme-Linked Immunosorbent Assay)<br> 4. Engvall, E., Perlmann, P. (1972): Enzyme-linked immunosorbent assay, ELISA. III.<br>EMITä (Enzyme Immunoassay Technique)<br> 5. Rubenstein, K.E., Schneider, R. S., Ullman, E.F. (1972): "Homogeneous" enzyme immunoassay. <br><br>Section II THERAPEUTIC DRUG MONITORING (TDM)<br>Bromide<br> 6. Wuth, O. (1927): Rational bromide treatment. <br>Sulfonamides<br> 7. Bratton, A.C., Marshall, E.K., Jr., (1939): A New Coupling Component for Sulfanilamide Determination. <br>Quinine and Quinidine<br> 8. Brodie, B.B., Udenfriend, S. (1943): The estimation of quinine in human plasma with a note on the estimation of quinidine. <br>Digoxin<br> 9. Smith, T.W., Butler, V.P., Haber, E. (1969): Determination of therapeutic and toxic serum digoxin concentrations by radioimmunoassay. <br>Theophylline<br> 10. Thompson, R.D., Nagasawa, H.T., Jenne, J.W. (1974): Determination of theophylline and its metabolites in human urine and serum by high-pressure liquid chromatography. <br><br>Section III ENZYMOLOGY<br>ALP (Alkaline Phosphatase, EC 3.1.3.1))<br> 11. Bessey, O.A., Lowry, O.H., Brock, M.J. (1946): A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum.<br>AST (Aspartate Aminotransferase, EC 2.6.1.1)<br> 12. Karmen, A., Wroblewski, F., LaDue, J.S. (1955): Transaminase activity in human blood. <br>CK (Creatine Kinase, EC 2.7.3.2)<br> 13. Mercer, D.W. (1974): Separation of tissue and serum creatine kinase isoenzymes by ion-exchange column chromatography. <br><br>Section IV SPECIFIC ANALYTES <br>Colorimetric Analysis with the Duboscq<br> 14. Folin, O., Wu, H. (1919): A system of blood analysis. <br>Blood Gases by Manometer<br> 15. Van Slyke, D.D. (1924): The determination of gases in blood and other solutions by vacuum extraction and manometric measurement. I. <br>Bilirubin, Total and Direct With the Filter Photometer<br> 16. Malloy, H.T., Evelyn, K.A. (1937): The determination of bilirubin with the photometric colorimeter.<br>Proteins by the Biuret Reaction<br> 17. Gornall, A. G., Bardawill, C. J., David, M. M. (1949): Determination of serum proteins by means of the Biuret reaction.<br>Methods for Pediatric and Neonatal Samples<br> 18. Natelson, S. (1951): Routine use of ultramicro methods in the clinical laboratory.<br>Enzymatic Glucose<br> 19. Huggett, A.S., Nixon, D.A. (1957): Use of glucose oxidase, peroxidase, and o-dianisidine in determination of blood and urinary glucose. <br>HCG (Human Chorionic Gonadotropin) by Immunoassay<br> 20. Wide, L., Gemzell, C.A. (1960): An immunological pregnancy test.<br>Hemoglobin A1c<br> 21. Rahbar, S. (1968): An Abnormal Hemoglobin in Red Cells of Diabetics. <br>LDL (Low Density Lipoprotein) Cholesterol by Calculation<br> 22. Friedewald, W.T., Levy, R.I., Fredrickson, D.S. (1972): Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge.<br><br>Section V INSTRUMENTATION AND TECHNIQUES<br>Paper Electrophoresis<br> 23. Durrum, E.L. (1950): A microelectrophoretic and microionophoretic technique. <br>Automated Analyzer<br> 24. Skeggs, L.T. (1957): An automatic method for colorimetric analysis. <br>Enzymatic Glucose, Dry Reagents on Pads<br> 25. Free, A.H., Adams, E.C., Kercher, M.L., Free, H. M., Cook, M. H., (1957): Simple Specific Test for Urine Glucose.<br>Blood Gas Elelctrodes<br> 26. Severinghaus, J., Bradley, A.F. (1958): Electrodes for blood pO2 and pCO2 determination. <br>Specific Ion Electrodes<br>27. Friedman, S.M., Nakashima, M. (1961): Single sample analysis with the sodium electrode.<br>Centrifugal Analyzer<br> 28. Anderson, N.G. (1969): Analytical techniques for cell fractions. XII. A multiple-cuvet rotor for a new microanalytical system. <br>Immunoassays on Membranes<br> 29. Glad, C., Grubb, A.O. (1978): Immunocapillary migration- A new method for immunochemical quantitation. <br>Western Blots<br> 30. Towbin, H., Stachelin, T., Gordon, J. (1979): Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications.<br>Capillary Electrophoresis (CE)<br> 31. Jorgenson, J.W., Lukacs, K. DeA. (1981): Zone electrophoresis in open-tubular glass capillaries. <br><br>Section VI CHEMOMETRICS<br>Quality Control Charts<br> 32. Levey, S., Jennings, E.R. (1950): The Use of Control Charts in the Clinical Laboratory.<br>Interferences in Analytical Accuracy<br> 33. Caraway, W. (1962): Chemical and Diagnostic Specificity of Laboratory Tests.<br>Predictive Value Theory<br> 34. Vecchio, T.J. (1966): Predictive value of a single diagnostic test in unselected populations. <br>Standards and Calibrators<br> 35. Radin, N. (1967): What is a standard?<br>Test Kits<br> 36. Barnett, R.N., Cash, A.D., Junghams, S.P. (1968): Performance of "kits" used for clinical chemical analysis of cholesterol. <br><br>Section VII MOLECULAR DIAGNOSTICS<br>Biochemical Genetics<br> 37. Pauling, L., Itano, H.A., Singer, S.J., Wells., I.C. (1949): Sickle cell anemia, a molecular disease. <br>Nucleic Acid Probes<br> 38. Hyypia, T., Jalava, A., Larsen, S.H., Terho, P., Hukkanene, V. (1985): Detection of Chlamydia trachomatis in clinical specimens by nucleic acid spot hybridization. <br>Microarrays<br> 39. Maskos, U., Southern, E.M. (1992): Oligonucleotide hybridizations on glass supports: A novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotide synthesized in situ. <br><br>Acknowledgments<br>Author Index<br>Subject Index