Yeast Protocols

 Use of Yeast Plasmids - Transformation and Inheritance Assays.- Construction of Tight Conditional Mutants Using the Improved Auxin-inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiae.- CRISPR Nickase-Mediated Base Editing in Yeast.- Genomic Promoter Shuffling by Using Recyclable Cassettes.- Study Essential Gene Functions by Plasmid Shuffling.- Scarless Genomic Protein Labeling in Saccharomyces cerevisiae.- High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae.- Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain Collections.- Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe.- Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-Map.- Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly.- Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE.- Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic Engineering.- A Versatile Protocol to Generate Translocations in Yeast Genomes using CRISPR/Cas9.- YeastNucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on Single Molecules of DNA.- Assays for Autophagy III: Observing Dynamic Protein Trafficking.- Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity Purification.- High-Throughput Rapid Yeast Chronological Lifespan Assay.- Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast.- Monitoring 5’-end Resection at Site-specific Double-strand Breaks by Southern Blot Analysis.