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Crop Breeding

Methods and Protocols

Specificaties
Gebonden, 255 blz. | Engels
Springer New York | 2014e druk, 2014
ISBN13: 9781493904457
Rubricering
Springer New York 2014e druk, 2014 9781493904457
Onderdeel van serie Methods in Molecular Biology
€ 180,99
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Samenvatting

The recent advent of molecular technologies has changed the way plant breeders identify and select their germplasm as genetic variation can now be assessed at the DNA level. Crop Breeding: Methods and Protocols presents detailed guidelines and tutorials that suit different needs and capacity from small laboratories analyzing molecular markers on a one-by-one basis to the increasingly popular high-throughput protocols for high capacity laboratories. Topics covered include breeding strategy for the selection of an ideal variety or genetic ideo type, protocols for breeders using molecular markers in selection programs and for laboratories providing molecular services to breeding programs, statistical programs and software to aid implementation of molecular data into breeding programs and methodologies that facilitate the generation of genetic diversity and its characterization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Crop Breeding: Methods and Protocols will help in expanding the use of molecular technologies for the creation of tomorrow’s crop varieties.

Specificaties

ISBN13:9781493904457
Taal:Engels
Bindwijze:gebonden
Aantal pagina's:255
Uitgever:Springer New York
Druk:2014

Inhoudsopgave

<p>1. Defining a Genetic Ideotype for Crop Improvement</p><p>                Richard M. Trethowan</p><p> </p><p>2. From Genes to Markers: Exploiting Gene Sequence Information to Develop Tools for Plant Breeding</p><p>                Melissa Garcia and Diane E. Mather</p><p> </p><p>3. Temperature Switch PCR (TSP) – A Gel-Based Molecular Marker Technique for Investigating Single Nucleotide Polymorphisms</p><p>                Thanh Le Phuoc and Kelvin Khoo</p><p> </p><p>4. Multiplex-Ready Technology for Mid-Throughput Genotyping of Molecular Markers</p><p>                Julien Bonneau and Matthew Hayden</p><p> </p><p>5. Genotyping by High-Resolution Melting Analysis</p><p>                Elise J. Tucker and Bao Lam Huynh</p><p> </p><p>6. Bi-Allelic SNP Genotyping Using the TaqMan® Assay</p><p>                John Woodward</p><p> </p><p>7. SNP Genotyping: The KASPTM Assay</p><p>                Chunlin He, John Holme, and Jeffrey Anthony</p><p> </p><p>8. Rindsel: An R Package for Phenotypic and Molecular Selection Indices Used in Plant Breeding</p><p>                S. Perez-Elizalde, J. J. Ceron-Rojas, J. Crossa, Delphine Fleury, and G. Alvarado</p><p> </p><p>9. OptiMAS: A Decision Support Tool to Conduct Marker-Assisted Selection Programs</p><p>                Fabio Valente, Franck Gauthier, Nicolas Bardol, Guylaine Blanc, Johann Joets, Alain Charcosset, and Laurence Moreau</p><p> </p><p>10. Genomic Selection in Plant Breeding</p><p>                M. A. Newell and J.-L Jannink</p><p> </p><p>11. Simulated Breeding with QU-GENE Graphical User Interface</p><p>                Adrian Hathorn, Sctoo Chapman, and Mark Dieters</p><p> </p><p>12. The Control of Recombination in Wheat by Ph1 and Its Use in Breeding</p><p>                Graham Moore</p><p> </p><p>13. TILLING for Plant Breeding</p><p>                Peter Sharp and Chongmei Dong</p><p> </p><p>14. In vitro Culture for Doubled Haploids: Tools for Molecular Breeding</p><p>                Sue Broughton, Parminder K. Sidhu, and Philip A. Davies</p><p> </p><p>15. Biolistic Transformation of Wheat with Centrophenoxine as a Synthetic Auxin</p><p>                Ainur Ismagul, Gulnur Oskakova, John C. Harris, and Serik Eliby</p><p> </p><p>16. Agrobacterium-Mediated Transformation of Barley (Hordeum vulgare L.)</p><p>                Ainur Ismagul, Iryna Mazonka, Corrine Callegari, and Serik Eliby</p><p> </p><p>17. qPCR for Quantification of Transgene Expression and Determination of Transgene Copy Number</p><p>                Stephen J. Fletcher</p><p> </p><p>18. High-Throughput Analysis Pipeline for Achieving Simple Low-Copy Wheat and Barley Transgenics</p><p>                Nataliya Kovalchuk</p>
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        Crop Breeding