<p>1. Journey of Human Gametes In Vitro: 1978 to 2010<br/>Zeev Shoham<br/>I. Setting Up and Running an IVF Laboratory<br/>2. Building the Laboratory<br/>Dean E. Morbeck and Marlena Duke<br/>3. Air Quality Management<br/>Johan Guns, Ronny Janssens, and Martine Vercammen<br/>4. Organizational Aspects of the Laboratory in a Tertiary Care ART Center<br/>Nancy L. Bossert and Christopher De Jonge<br/>5. Quality Control Management<br/>William R. Boone and H. Lee Higdon III<br/>6. Daily, Weekly, and Regular Preparations for the IVF Laboratory<br/>Michael A. Britt and Klaus E. Wiemer <br/>7. Risk and Safety in the IVF Clinic<br/>Peter Sjoblom and Julius Hreinsson<br/>II. IVF Laboratory Equipment and Culture Systems<br/>8. Essential Instruments and Disposable Supplies for an IVF Laboratory <br/>Leslie Weikert, Christa Fralick, and Klaus E. Wiemer<br/>9. Co2 and Low-O2 Incubators<br/>Marius Meintjes<br/>10. IVF Workstations<br/>Nicolas Prados and Alex C. Varghese<br/>11. Culture Media in IVF: Decisions for the Laboratory<br/>Jason E. Swain and Thomas B. Pool <br/>III. In Vitro Fertilization<br/>12. Oocyte Denuding<br/>Roberta Maggiulli, Filippo Ubaldi, and Laura Rienzi<br/>13. Assessment of Oocyte Quality <br/>Basak Balaban, Turgay Barut, and Bülent Urman <br/>14. Polarization Microscopy<br/>Markus Montag, Maria Köster, and Hans van der Ven<br/>15. Cumulus Cell Gene Expression in Assessment of Oocyte Quality <br/>Dagan Wells<br/>IV. Embryo Culture Methods<br/>16. Short Culture: Day 1/Day 2/Day 3 Embryo Culture<br/>Patrick Quinn<br/>17. Extended Culture in IVF<br/>David K. Gardner and Michelle Lane<br/>18. In Vitro Maturation of Human Oocytes <br/>Baris Ata, Jack Huang, and Ri-Cheng Chian<br/>19. In Vivo Embryo Culture Device<br/>Claude Ranoux<br/>20. Microfluidics for Gamete Manipulation and Embryo Culture<br/>Gary D. Smith, Charles Bormann, and Shuichi Takayama<br/>V. Sperm Processing and Selection<br/>21. Sperm Assessment: Traditional Approaches and Their Indicative Value <br/>Margot Flint, Fanuel Lampiao, Ashok Agarwal, and Stefan S du Plessis<br/>22. Sperm Assessment: Novel Approaches and Their Indicative Value<br/>De Y. Liu, Harold Bourne, C. Garrett, G. N. Clarke, Shlomi Barak, and H. W. G. Baker<br/>23. Sperm Processing for IVF<br/>Ralf Henkel<br/>24. PESA/TESA/TESE Sperm Processing <br/>Sandro C. Esteves and Sidney Verza, Jr.<br/>25. Processing Sperm Samples in HIV-Positive Patients<br/>Thamara Viloria Samochin, Marcos Meseguer, Antonio Pellicer, José Remohí, and Nicolás Garrido<br/>26. Intracytoplasmic Morphologically Selected Sperm Injection<br/>P. Vanderzwalmen, Magnus Bach, Batsuren Baramsai, A. Neyer, Delf Schwerda, Astrid Stecher, Barbara Wirleitner, Martin Zintz, Bernard Lejeune, S. Vanderzwalmen, Nino Guy Cassuto, Mathias Zech, and Nicolas H. Zech <br/>27. Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in the Andrology and IVF Laboratories <br/>Gabor Huszar<br/>28. Electrophoretic Sperm Separation<br/>Steven Fleming and John Aitken <br/>29. Magnetic Activated Cell Sorting of Human Spermatozoa<br/>Enver Kerem Dirican<br/>30. Poloscope-Based Sperm Selection <br/>Luca Gianaroli, M. Cristina Magli, Andor Crippa, Giorgio Cavallini, Eleonora Borghi, and Anna P. Ferraretti<br/>VI. Insemination Procedures<br/>31. Intrauterine Insemination <br/>Gautam N. Allahbadia and Rubina Merchant <br/>32. Conventional IVF Insemination<br/>Liesl Nel-Themaat, Thomas Elliott, Ching-Chien Chang, Graham Wright, and Zsolt Peter Nagy<br/>33. Intracytoplasmic Sperm Injection <br/>Gianpiero D. Palermo, Queenie V. Neri, Devin Monahan, Takumi Takeuchi, Peter N. Schlegel, and Zev Rosenwaks<br/>34. Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed Fertilization<br/>Dmitri Dozortsev and Mohammad Hossein Nasr-Esfahani <br/>VII. Micromanipulators and Micromanipulation<br/>35. Hydraulic Manipulators for ICSI<br/>Hubert Joris<br/>36. Research Instruments Micromanipulators<br/>Steven Fleming and Catherine Pretty<br/>37. Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators<br/>Ehab Abu-Marar and Safa Al-Hasani<br/>38. The Leica-IMSI System<br/>Christiane Wittemer, Bruno Laborde, Frederic Ribay, and Stephane Viville <br/>39. Automated Robotic Intracytoplasmic Sperm Injection<br/>Zhe Lu, Xinyu Liu, Xuping Zhang, Clement Leung, Navid Esfandiari, Robert F. Casper, and Yu Sun<br/>40. Oocyte Treatment and Preparation for Microinjection <br/>Thomas Ebner <br/>41. Livestock Production Via Micromanipulation<br/>Akira Onishi and Anthony C. F. Perry<br/>VIII. Embryo Evaluation, Grading, and Assisted Hatching<br/>42. Pronuclear Scoring in Human In Vitro Fertilization<br/>Lynette Scott<br/>43. Cumulative Morphological Assessment of Embryo Quality<br/>Barry Behr and Aparna Hegde<br/>44. Metabolomics: The ViaMetrics-E™ Procedure for Assessing Embryo Viability<br/>D. Sakkas, L. Botros, M. Henson, K. Judge, and P. Roos<br/>45. Oxygen Consumption as an Indicator of Oocyte and Embryo Viability<br/>Ana S. Lopes<br/>46. Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection<br/>Samir Hamamah, Said Assou, Imène Boumela, Gisèle Ouandaogo, Hervé Dechaud, and John de Vos<br/>47. Amino Acid Turnover as a Biomarker of Embryo Viability<br/>Roger Sturmey, Christine Leary, Henry Leese, and Danielle Smith<br/>48. Real-time Embryo Monitoring Devices for Embryo Selection<br/>Gábor Vajta and Thorir Hardarson<br/>49. Assisted Hatching in IVF<br/>Itziar Belil and Anna Veiga<br/>IX. Biopsy Procedures on Oocytes and Embryos<br/>50. Polar Body Biopsy<br/>Markus Montag, Maria Köster, K. van der Ven, and Hans van der Ven<br/>51. Cleavage Stage Embryo Biopsy<br/>Alan R. Thornhill<br/>52. Embryo Biopsy for PGD: Current Perspective<br/>Steven J. McArthur, Don Leigh, Maria Traversa, James Marshall, and Robert P. S. Jansen <br/>53. Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects<br/>Gary Harton and Santiago Munné<br/>X. Cryopreservation<br/>54. Sperm Cryopreservation<br/>Fabio Firmbach Pasqualotto, Eleonora Bedin Pasqualotto, Edson Borges, Jr., and Ashok Agarwal<br/>55. Slow Freezing of Oocytes<br/>Giovanni Coticchio and Lucia De Santis<br/>56. Vitrification: Research in Animal Models<br/>Gábor Vajta<br/>57. Oocyte Vitrification<br/>Ana Cobo<br/>58. Slow Freezing of Embryos<br/>Liesl Nel-Themaat, Ching-Chien Chang, Thomas Elliott, Patricia Bernal, Graham Wright, and Zsolt Peter Nagy<br/>59. Vitrification of Embryos<br/>Juergen Liebermann, Joe Conaghan, Zsolt Peter Nagy, and Michael Tucker<br/>60. Directional Freezing of Reproduction Cells, Tissues, and Organs<br/>Amir Arav<br/>61. Ovarian Tissue Cryopreservation<br/>Jennifer L Kulp, J. Ryan Martin, and Pasquale Patrizio<br/>XI. Embryo Transfer<br/>62. Assessment of Uterine Receptivity<br/>Hakan Cakmak and Hugh S. Taylor<br/>63. Single Embryo Transfer <br/>Thorir Hardarson and Matts Wikland<br/>64. Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-Based Evaluation<br/>Ahmed M. Abou-Setta<br/>65. Cumulus-Aided Embryo Transfer<br/>Firuza R. Parikh, Nandkishor J. Naik, Dattatray J. Naik, and Dhanajaya Kulkarni<br/>XII. Management and Regulation in the ART Laboratory<br/>66. Data Management in the ART Laboratory: Requirements and Solutions<br/>Timothy Brown and Bruce R. Gilbert<br/>67. Regulation, Licensing, and Accreditation of the ART Laboratory<br/>Doris Baker<br/>68. Legislation in the United Kingdom<br/>Rachel Cutting<br/>69. Regulation, Licensing, and Accreditation of the ART Laboratory in Europe<br/>Julius Hreinsson and Peter Sjoblom<br/>70. Regulation, Licensing, and Accreditation of ART Laboratories in India <br/>B. N. Chakravarty and Rita Modi<br/>71. Troubleshooting in the Clinical Embryology Laboratory: The Art of Problem-Solving in ART<br/>Kathryn J. Go, Jay C. Patel, and Rick Dietz<br/>XIII. Special Topics<br/>72. The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development<br/>Jonathan Van Blerkom<br/>73. Nuclear and Cytoplasmic Transfer: Human Applications and Concerns<br/>Josef Fulka, Jr. and Helena Fulka<br/>74. Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization<br/>Heide Schatten, Vanesa Y. Rawe, and Qing-Yuan Sun<br/>75. Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence<br/></p>